Isolation and identification macrocyclic spermine alkaloid(protoverbine) from verbascum speciosum.

DNA isolation from small tissue samples using salt and spermine.

Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. These solvents pose some risk to the user and require special disposal procedures. We previously reported a method for isolating DNA from peripheral blood lymphocytes by precipitation with spermine (1). This procedure proved less effective with other tissues, especially lung, and the s...

ISOLATION AND IDENTIFICATION OF IRONSULPHUR OXIDZING THIOBACIULLI FROM IRAN ENVRONMENTS

Two hundred samples were taken from various sites in Iran, including coal storage areas, waste water in the Tabas and Zirab coal mines, and sulphur containing acid springs in Ramsar and Abask , and were screened for Thlobacillus ferruuxidans. During this investigation , seven strains were isolated which could oxidize sdphm , pyrite , thiosdphate and ferrous iron as the sole energy source. Macr...

Isolation and identification of halophilicbacteria from Urmia Lake in Iran

Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to form major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake fr...

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Isolation and identification of Clostridium septicum from sheep-dung

  To study the existence of Clostridium septicum as probable agent associated with currently occurrence of black quarter in Urmia area, sheep-dung samples were collected. The bacteriological examinations on suspicious gram positive anaerobic spore-bearing bacilli which are in Clostridia genus include isolation and biochemical identification were performed. The results showed 28 different types ...

Isolation and identification of halophilicbacteria from Urmia Lake in Iran

Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to form major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake fr...